Rabbit anti-IKB alpha (pS32/S36) Antibody
Product Specifications
Background
Rabbit polyclonal antibody to IKB alpha (pS32/S36)
Synonyms
IKBA; MAD3; NFKBI; NF-kappa-B inhibitor alpha; I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3
Host
Rabbit
Reactivity
H, M, R, B, P, S
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human IKB alpha. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of IKB alpha (pS32/S36) expression in HepG2 TNFa-treated (A), Raw264.7 TNFa-treated (B), PC12 TNFa-treated (C) whole cell lysates.
IHC description
Immunohistochemical analysis of IKB alpha (pS32/S36) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of IKB alpha (pS32/S36) staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
4792
Gene ID (Mouse)
18035
Gene ID (Rat)
25493
SwissProt (Human)
P25963
SwissProt (Mouse)
Q9Z1E3
SwissProt (Rat)
Q63746
CAS Number
9007-83-4
Available Sizes
Curated Selection
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