Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

Rabbit anti-HSP90 beta (pS226) Antibody

Product Specifications

Background

Rabbit polyclonal antibody to HSP90 beta (pS226)

Synonyms

HSP90B; HSPC2; HSPCB; Heat shock protein HSP 90-beta; HSP 90; Heat shock 84 kDa; HSP 84; HSP84

Host

Rabbit

Reactivity

H, M, R, B, Mk

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HSP90 beta. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1 mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of HSP90 beta (pS226) expression in L929 (A), Raw264.7 (B) whole cell lysates.

IHC description

Immunohistochemical analysis of HSP90 beta (pS226) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of HSP90 beta (pS226) staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .

Gene ID (Human)

3326

Gene ID (Mouse)

15516

Gene ID (Rat)

301252

SwissProt (Human)

P08238

SwissProt (Mouse)

P11499

SwissProt (Rat)

P34058

CAS Number

9007-83-4

Available Sizes

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

Rabbit Anti Human HIST1H1C Monoclonal Clone AEDF-8 100UL
IRBAHUHIST1H1CAEDF8C100UL 100 µL

Rabbit Anti Human HIST1H1C Monoclonal Clone AEDF-8 100UL

Ask
View Details
CTNNBL1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV)
17076061 1.0 µg DNA

CTNNBL1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV)

Ask
View Details
PHF21B Double Nickase Plasmid (m2)
sc-435441-NIC-2 20 µg

PHF21B Double Nickase Plasmid (m2)

Ask
View Details
Rabbit Polyclonal Erythropoietin R Antibody
NBP1-19388 0.1 mL

Rabbit Polyclonal Erythropoietin R Antibody

Ask
View Details
Cers1 (NM_138647) Mouse Tagged Lenti ORF Clone
MR221078L4 10 µg

Cers1 (NM_138647) Mouse Tagged Lenti ORF Clone

Ask
View Details