Rabbit anti-HSP27 (pS82) Antibody
Product Specifications
Background
Rabbit polyclonal antibody to HSP27 (pS82)
Synonyms
HSP27; HSP28; Heat shock protein beta-1; HspB1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27
Host
Rabbit
Reactivity
H, M, B, D
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HSP27 (pS82) . The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of HSP27 (pS82) expression in A549 (A), HepG2 (B), K562 (C) whole cell lysates.
IHC description
Immunohistochemical analysis of HSP27 (pS82) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
3315
Gene ID (Mouse)
15507
SwissProt (Human)
P04792
SwissProt (Mouse)
P14602
CAS Number
9007-83-4
Available Sizes
Curated Selection
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