Rabbit anti-HSP27 (pS82) Antibody

Product Specifications

Background

Rabbit polyclonal antibody to HSP27 (pS82)

CAS Number

9007-83-4

Synonyms

HSP27; HSP28; Heat shock protein beta-1; HspB1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27

Host

Rabbit

Reactivity

H, M, B, D

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HSP27 (pS82) . The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of HSP27 (pS82) expression in A549 (A), HepG2 (B), K562 (C) whole cell lysates.

IHC description

Immunohistochemical analysis of HSP27 (pS82) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.