Rabbit anti-HSD3B7 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to HSD3B7
CAS Number
9007-83-4
Synonyms
3 beta-hydroxysteroid dehydrogenase type 7; 3 beta-hydroxysteroid dehydrogenase type VII; 3-beta-HSD VII; 3-beta-hydroxy-Delta (5) -C27 steroid oxidoreductase; C (27) 3-beta-HSD; Cholest-5-ene-3-beta,7-alpha-diol 3-beta-dehydrogenase
Host
Rabbit
Reactivity
H, M, R
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HSD3B7. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
Host or Source
Rabbit
WB Description
Western blot analysis of HSD3B7 expression in Lovo (A), MCF7 (B), Hela (C) whole cell lysates.
IHC description
Immunohistochemical analysis of HSD3B7 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
80270
Gene ID (Mouse)
101502
Gene ID (Rat)
246211
SwissProt (Human)
Q9H2F3
SwissProt (Mouse)
Q9EQC1
SwissProt (Rat)
O35048
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