Rabbit anti-H-Ras Antibody
Product Specifications
Background
Rabbit polyclonal antibody to H-Ras
Synonyms
HRAS1; GTPase HRas; H-Ras-1; Ha-Ras; Transforming protein p21; c-H-ras; p21ras
Host
Rabbit
Reactivity
H
Immunogen
KLH-conjugated synthetic peptide of human H-Ras
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of H-Ras expression in SKOV3 (A), mouse kidney (B), rat brain (C) whole cell lysates.
IHC description
Immunohistochemical analysis of H-Ras staining in human gastric cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
3265
SwissProt (Human)
P01112
CAS Number
9007-83-4
Available Sizes
Curated Selection
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