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Rabbit anti-hnRNP F Antibody

Product Specifications

Background

Rabbit polyclonal antibody to hnRNP F

Synonyms

HNRPF; Heterogeneous nuclear ribonucleoprotein F; hnRNP F; Nucleolin-like protein mcs94-1

Host

Rabbit

Reactivity

H, M, R, B, Mk

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human hnRNP F. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1 mg/mL

Dilution

WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of hnRNP F expression in HepG2 (A), Jurkat (B) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of hnRNP F staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

3185

Gene ID (Mouse)

98758

Gene ID (Rat)

64200

SwissProt (Human)

P52597

SwissProt (Mouse)

Q9Z2X1

SwissProt (Rat)

Q794E4

CAS Number

9007-83-4

Available Sizes

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