Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

Rabbit anti-HNF4 alpha/gamma Antibody

Product Specifications

Background

Rabbit polyclonal antibody to HNF4 alpha/gamma

Synonyms

HNF4A; HNF4; NR2A1; TCF14; Hepatocyte nuclear factor 4-alpha; HNF-4-alpha; Nuclear receptor subfamily 2 group A member 1; Transcription factor 14; TCF-14; Transcription factor HNF-4; HNF4G; NR2A2; Hepatocyte nuclear factor 4-gamma; HNF-4-gamma; Nuclear receptor subfamily 2 group A member 2

Host

Rabbit

Reactivity

H, M, R

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HNF4 alpha/gamma. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1 mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of HNF4 alpha/gamma expression in A549 (A), HepG2 (B), Hela (C) whole cell lysates.

IHC description

Immunohistochemical analysis of HNF4 alpha/gamma staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of HNF4 alpha/gamma staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

3172; 3174

Gene ID (Mouse)

15378; 30942

Gene ID (Rat)

25735

SwissProt (Human)

P41235; Q14541

SwissProt (Mouse)

P49698; Q9WUU6

SwissProt (Rat)

P22449

CAS Number

9007-83-4

Available Sizes

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

N-Nitrosopiperazine
TRC-N545350-500MG 500 mg

N-Nitrosopiperazine

Ask
View Details
POTEJ Human Gene Knockout Kit (CRISPR)
KN435135 1 Kit

POTEJ Human Gene Knockout Kit (CRISPR)

Ask
View Details
Csf1 (NM_007778) Mouse Recombinant Protein
PM42512M5 20 µg

Csf1 (NM_007778) Mouse Recombinant Protein

Ask
View Details
LFNG Adenovirus (Human)
26459051 1.0 ml

LFNG Adenovirus (Human)

Ask
View Details
Ehhadh Rat siRNA Oligo Duplex (Locus ID 171142)
SR508696 1 Kit

Ehhadh Rat siRNA Oligo Duplex (Locus ID 171142)

Ask
View Details
FBXW7 CRISPRa sgRNA lentivector (set of three targets)(Human)
20370121 3 x 1.0μg DNA

FBXW7 CRISPRa sgRNA lentivector (set of three targets)(Human)

Ask
View Details