Rabbit anti-Histone H4 (pS47) Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Histone H4 (pS47)
Synonyms
H4/A; H4FA; H4/I; H4FI; H4/G; H4FG; H4/B; H4FB; H4/J; H4FJ; H4/C; H4FC; H4/H; H4FH; H4/M; H4FM; H4/E; H4FE; H4/D; H4FD; H4/K; H4FK; H4/N; H4F2; H4FN; HIST2H4; H4/O; H4FO; Histone H4
Host
Rabbit
Reactivity
H, M, R
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence of human Histone H4. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/1000 - 1/2000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Histone H4 (pS47) expression in Hela Nocodazole-treated (A) whole cell lysates.
IHC description
Immunohistochemical analysis of Histone H4 (pS47) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
121504
Gene ID (Mouse)
100041230
Gene ID (Rat)
100360950
SwissProt (Human)
P62805
SwissProt (Mouse)
P62806
SwissProt (Rat)
P62804
CAS Number
9007-83-4
Available Sizes
Curated Selection
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