Rabbit anti-Histone H1 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Histone H1
Synonyms
HIST1H1A; H1F1; Histone H1.1; Histone H1a; HIST1H1C; H1F2; Histone H1.2; Histone H1c; Histone H1d; Histone H1s-1; HIST1H1D; H1F3; Histone H1.3; Histone H1c; Histone H1s-2; HIST1H1E; H1F4; Histone H1.4; Histone H1b; Histone H1s-4
Host
Rabbit
Reactivity
H, M
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence of human Histone H1. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/1000 - 1/2000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Histone H1 expression in Hela (A), NIH3T3 (B) whole cell lysates.
IHC description
Immunohistochemical analysis of Histone H1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
3024; 3006; 3007; 3008
Gene ID (Mouse)
80838; 50708; 14957; 50709
SwissProt (Human)
Q02539; P16403; P16402; P10412
SwissProt (Mouse)
P43275; P15864; P43277; P43274
CAS Number
9007-83-4
Available Sizes
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