Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

Rabbit anti-HER3 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to HER3

Synonyms

HER3; Receptor tyrosine-protein kinase erbB-3; Proto-oncogene-like protein c-ErbB-3; Tyrosine kinase-type cell surface receptor HER3

Host

Rabbit

Reactivity

H, M, R

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human HER3. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1 mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of HER3 expression in HEK293T (A), Hela (B) whole cell lysates.

IHC description

Immunohistochemical analysis of HER3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of HER3 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .

Gene ID (Human)

2065

Gene ID (Mouse)

13867

Gene ID (Rat)

29496

SwissProt (Human)

P21860

SwissProt (Mouse)

Q61526

SwissProt (Rat)

Q62799

CAS Number

9007-83-4

Available Sizes

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

Istradefylline Impurity 15
RM-I190415-01 10 mg

Istradefylline Impurity 15

Ask
View Details
Istradefylline Impurity 15
RM-I190415-02 25 mg

Istradefylline Impurity 15

Ask
View Details
Istradefylline Impurity 15
RM-I190415-03 50 mg

Istradefylline Impurity 15

Ask
View Details
Istradefylline Impurity 15
RM-I190415-04 100 mg

Istradefylline Impurity 15

Ask
View Details
Recombinant Ustilago maydis RuvB-like helicase 1 (RVB1)
MBS1359594-01 0.02 mg (E-Coli)

Recombinant Ustilago maydis RuvB-like helicase 1 (RVB1)

Ask
View Details
Recombinant Ustilago maydis RuvB-like helicase 1 (RVB1)
MBS1359594-02 0.02 mg (Yeast)

Recombinant Ustilago maydis RuvB-like helicase 1 (RVB1)

Ask
View Details