Rabbit anti-HADHB Antibody
Product Specifications
Background
Rabbit polyclonal antibody to HADHB
Synonyms
Trifunctional enzyme subunit beta, mitochondrial; TP-beta
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human HADHB
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/10 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of HADHB expression in HepG2 (A), Jurkat (B), mouse liver (C), rat liver (D) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of HADHB staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
3032
Gene ID (Mouse)
231086
Gene ID (Rat)
171155
SwissProt (Human)
P55084
SwissProt (Mouse)
Q99JY0
SwissProt (Rat)
Q60587
CAS Number
9007-83-4
Available Sizes
Curated Selection
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