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Rabbit anti-GTL3 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to GTL3

Synonyms

UPF0468 protein C16orf80; Transcription factor IIB

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human GTL3

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1 mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/50 - 1/100)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of GTL3 expression in SKOV3 (A), HepG2 (B), mouse liver (C), rat lung (D) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of GTL3 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

29105

Gene ID (Mouse)

14894

Gene ID (Rat)

307642

SwissProt (Human)

Q9Y6A4

SwissProt (Mouse)

Q8BTU1

SwissProt (Rat)

Q499T7

CAS Number

9007-83-4

Available Sizes

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