Rabbit anti-GSPT1 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to GSPT1
CAS Number
9007-83-4
Synonyms
ERF3A; Eukaryotic peptide chain release factor GTP-binding subunit ERF3A; Eukaryotic peptide chain release factor subunit 3a; eRF3a; G1 to S phase transition protein 1 homolog
Host
Rabbit
Reactivity
H, M, Z
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GSPT1. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
Host or Source
Rabbit
WB Description
Western blot analysis of GSPT1 expression in Hela (A), HepG2 (B), NIH3T3 (C) whole cell lysates.
IHC description
Immunohistochemical analysis of GSPT1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
2935
Gene ID (Mouse)
14852
SwissProt (Human)
P15170
SwissProt (Mouse)
Q8R050
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