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Rabbit anti-GRP170 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to GRP170

CAS Number

9007-83-4

Synonyms

GRP170; ORP150; Hypoxia up-regulated protein 1; 150 kDa oxygen-regulated protein; ORP-150; 170 kDa glucose-regulated protein; GRP-170

Host

Rabbit

Reactivity

H, M

Immunogen

Recombinant full length protein of human GRP170

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of GRP170 expression in MCF7 (A), mouse pancreas (B) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of GRP170 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

10525

Gene ID (Mouse)

12282

SwissProt (Human)

Q9Y4L1

SwissProt (Mouse)

Q9JKR6

Available Sizes

Frequently Asked Questions

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