Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

Rabbit anti-GPx-4 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to GPx-4

CAS Number

9007-83-4

Synonyms

Phospholipid hydroperoxide glutathione peroxidase, mitochondrial; PHGPx; Glutathione peroxidase 4; GPx-4; GSHPx-4

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human GPx-4

Clonality

Polyclonal

Applications

WB, IHC, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/10 - 1/100)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of GPx-4 expression in mouse kidney (A) whole cell lysates.

IHC description

Immunohistochemical analysis of GPx-4 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of GPx-4 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

2879

SwissProt (Human)

P36969

Available Sizes

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

Wbp5-set siRNA/shRNA/RNAi Lentivector (Rat)
50279096 4x 500 ng

Wbp5-set siRNA/shRNA/RNAi Lentivector (Rat)

Ask
View Details
Human H2BC18 (NM_001024599) AAV Particle
RC211042A1V 250 µL

Human H2BC18 (NM_001024599) AAV Particle

Ask
View Details
Recombinant Human TMEFF1 Protein, His, E.coli-1mg
QP13762-1mg 1mg

Recombinant Human TMEFF1 Protein, His, E.coli-1mg

Ask
View Details
BHMT (Betaine-homocysteine methyltransferase, BHMT)
B1082-13 100 µg

BHMT (Betaine-homocysteine methyltransferase, BHMT)

Ask
View Details
Arfrp1 Mouse Gene Knockout Kit (CRISPR)
KN501482 1 Kit

Arfrp1 Mouse Gene Knockout Kit (CRISPR)

Ask
View Details
Rabbit Polyclonal MUPP1 Antibody [Alexa Fluor 700]
NBP1-49975AF700 0.1 mL

Rabbit Polyclonal MUPP1 Antibody [Alexa Fluor 700]

Ask
View Details