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Rabbit anti-GPT Antibody

Product Specifications

Background

Rabbit polyclonal antibody to GPT

Synonyms

AAT1; GPT1; Alanine aminotransferase 1; ALT1; Glutamate pyruvate transaminase 1; GPT 1; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human GPT

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1 mg/mL

Dilution

WB (1/500 - 1/2000), IH (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of GPT expression in mouse lung (A), rat liver (B), rat heart (C) whole cell lysates.

IHC description

Immunohistochemical analysis of GPT staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

2875

Gene ID (Mouse)

76282

Gene ID (Rat)

81670

SwissProt (Human)

P24298

SwissProt (Mouse)

Q8QZR5

SwissProt (Rat)

P25409

CAS Number

9007-83-4

Available Sizes

Curated Selection

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