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Rabbit anti-GPRC6A Antibody

Product Specifications

Background

Rabbit polyclonal antibody to GPRC6A

Synonyms

G-protein coupled receptor family C group 6 member A; hGPRC6A; G-protein coupled receptor GPCR33; hGPCR33

Host

Rabbit

Reactivity

H, M, R

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GPRC6A. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1 mg/mL

Dilution

WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of GPRC6A expression in HEK293T (A), LO2 (B), rat testis (C) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of GPRC6A staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

222545

Gene ID (Mouse)

210198

Gene ID (Rat)

294394

SwissProt (Human)

Q5T6X5

SwissProt (Mouse)

Q8K4Z6

SwissProt (Rat)

Q70VB1

CAS Number

9007-83-4

Available Sizes

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