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Rabbit anti-GPR66 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to GPR66

CAS Number

9007-83-4

Synonyms

GPR66; Neuromedin-U receptor 1; NMU-R1; G-protein coupled receptor 66; G-protein coupled receptor FM-3

Host

Rabbit

Reactivity

H, M, R

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human GPR66. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of GPR66 expression in rat testis (A), mouse testis (B), SGC7901 (C), K562 (D) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of GPR66 staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

10316

Gene ID (Mouse)

14767

Gene ID (Rat)

65276

SwissProt (Human)

Q9HB89

SwissProt (Mouse)

O55040

SwissProt (Rat)

Q9JJI5

Available Sizes

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