Rabbit anti-GM130 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to GM130
Synonyms
Golgin subfamily A member 2; 130 kDa cis-Golgi matrix protein; GM130; GM130 autoantigen; Golgin-95
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human GM130
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of GM130 expression in Raji (A), Hela (B) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of GM130 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
2801
Gene ID (Mouse)
99412
Gene ID (Rat)
64528
SwissProt (Human)
Q08379
SwissProt (Mouse)
Q921M4
SwissProt (Rat)
Q62839
CAS Number
9007-83-4
Available Sizes
Curated Selection
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