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Rabbit anti-Glutathione Synthetase Antibody

Product Specifications

Background

Rabbit polyclonal antibody to Glutathione Synthetase

Synonyms

Glutathione synthetase; GSH synthetase; GSH-S; Glutathione synthase

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human Glutathione Synthetase

Clonality

Polyclonal

Applications

WB, IHC, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1 mg/mL

Dilution

WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of Glutathione Synthetase expression in SW480 (A), K562 (B), Raji (C), mouse kidney (D), mouse liver (E) whole cell lysates.

IHC description

Immunohistochemical analysis of Glutathione Synthetase staining in mouse lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of Glutathione Synthetase staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

2937

Gene ID (Mouse)

14854

Gene ID (Rat)

25458

SwissProt (Human)

P48637

SwissProt (Mouse)

P51855

SwissProt (Rat)

P46413

CAS Number

9007-83-4

Available Sizes

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