Rabbit anti-GBL Antibody
Product Specifications
Background
Rabbit polyclonal antibody to GBL
CAS Number
9007-83-4
Synonyms
GBL; LST8; Target of rapamycin complex subunit LST8; TORC subunit LST8; G protein beta subunit-like; Gable; Protein GbetaL; Mammalian lethal with SEC13 protein 8; mLST8
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human GBL
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/10 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
Host or Source
Rabbit
WB Description
Western blot analysis of GBL expression in K562 (A), HepG2 (B), COS7 (C), mouse testis (D), mouse spleen (E) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of GBL staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
64223
Gene ID (Mouse)
56716
Gene ID (Rat)
64226
SwissProt (Human)
Q9BVC4
SwissProt (Mouse)
Q9DCJ1
SwissProt (Rat)
Q9Z2K5
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