Rabbit anti-GADD45 alpha Antibody
Product Specifications
Background
Rabbit polyclonal antibody to GADD45 alpha
Synonyms
DDIT1; GADD45; Growth arrest and DNA damage-inducible protein GADD45 alpha; DNA damage-inducible transcript 1 protein; DDIT-1
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human GADD45 alpha
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/10 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
Host or Source
Rabbit
WB Description
Western blot analysis of GADD45 alpha expression in mouse liver (A), mouse kidney (B) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of GADD45 alpha staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
1647
Gene ID (Mouse)
13197
SwissProt (Human)
P24522
SwissProt (Mouse)
P48316
SwissProt (Rat)
P48317
CAS Number
9007-83-4
Available Sizes
Curated Selection
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