Rabbit anti-GAD2 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to GAD2
Synonyms
GAD65; Glutamate decarboxylase 2; 65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 65 kDa isoform
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human GAD2
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/10 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of GAD2 expression in mouse heart (A), mouse brain (B) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of GAD2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
2572
Gene ID (Mouse)
14417
Gene ID (Rat)
24380
SwissProt (Human)
Q05329
SwissProt (Mouse)
P48320
SwissProt (Rat)
Q05683
CAS Number
9007-83-4
Available Sizes
Curated Selection
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