Rabbit anti-GABPB1 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to GABPB1
Synonyms
E4TF1B; GABPB; GABPB2; GA-binding protein subunit beta-1; GABP subunit beta-1; GABPB-1; GABP subunit beta-2; GABPB-2; Nuclear respiratory factor 2; Transcription factor E4TF1-47; Transcription factor E4TF1-53
Host
Rabbit
Reactivity
H, M
Immunogen
Recombinant full length protein of human GABPB1
Clonality
Polyclonal
Applications
WB, IHC, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of GABPB1 expression in HepG2 (A), HT29 (B), mouse spleen (C), mouse thymus (D) whole cell lysates.
IHC description
Immunohistochemical analysis of GABPB1 staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of GABPB1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
2553
Gene ID (Mouse)
14391
SwissProt (Human)
Q06547
SwissProt (Mouse)
Q00420
CAS Number
9007-83-4
Available Sizes
Curated Selection
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