Rabbit anti-FOXO1/3 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to FOXO1/3
Synonyms
FOXO1; FKHR; FOXO1A; Forkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma; FOXO3; FKHRL1; FOXO3A; Forkhead box protein O3; AF6q21 protein; Forkhead in rhabdomyosarcoma-like 1
Host
Rabbit
Reactivity
H, M, R, B, P
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human FOXO1/3. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of FOXO1/3 expression in A549 (A), RAW264.7 (B), PC12 (C) whole cell lysates.
IHC description
Immunohistochemical analysis of FOXO1/3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
2308
Gene ID (Mouse)
56458; 56484
Gene ID (Rat)
84482
SwissProt (Human)
Q12778; O43524
SwissProt (Mouse)
Q9R1E0; Q9WVH4
SwissProt (Rat)
G3V7R4
CAS Number
9007-83-4
Available Sizes
Curated Selection
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