Rabbit anti-Focal Adhesion Kinase (pY925) Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Focal Adhesion Kinase (pY925)
CAS Number
9007-83-4
Synonyms
FAK; FAK1; Focal adhesion kinase 1; FADK 1; Focal adhesion kinase-related nonkinase; FRNK; Protein phosphatase 1 regulatory subunit 71; PPP1R71; Protein-tyrosine kinase 2; p125FAK; pp125FAK
Host
Rabbit
Reactivity
H, M, R, C, Z
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Focal Adhesion Kinase. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
Host or Source
Rabbit
WB Description
Western blot analysis of Focal Adhesion Kinase (pY925) expression in NIH3T3 pervanadate-treated (A) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of Focal Adhesion Kinase (pY925) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
5747
Gene ID (Mouse)
14083
Gene ID (Rat)
25614
SwissProt (Human)
Q05397
SwissProt (Mouse)
P34152
SwissProt (Rat)
O35346
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
BNC400017-100 1x 100 µL
Ep-CAM / CD326 (VU-1D9), CF640R conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC680204-100 1x 100 µL
Complement C4d (C4D204), CF568 conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC741073-100 1x 100 µL
HLA-Aw32 & HLA-A25 (MHC I) (CATA-1), CF740 conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC680010-500 1x 500 µL
MART-1 / Melan-A (M2-9E3), CF568 conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC680061-500 1x 500 µL
Granulocyte Marker (BM-1), CF568 conjugate, 0.1mg/mL
Sign In for Pricing
View Details