Rabbit anti-FKBP6 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to FKBP6
Synonyms
FKBP36; Inactive peptidyl-prolyl cis-trans isomerase FKBP6; Inactive PPIase FKBP6; 36 kDa FK506-binding protein; 36 kDa FKBP; FKBP-36; FK506-binding protein 6; FKBP-6; Immunophilin FKBP36
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human FKBP6
Clonality
Polyclonal
Applications
WB, IHC, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of FKBP6 expression in HepG2 (A), mouse heart (B), mouse liver (C), rat testis (D) whole cell lysates.
IHC description
Immunohistochemical analysis of FKBP6 staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of FKBP6 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
8468
Gene ID (Mouse)
94244
Gene ID (Rat)
288597
SwissProt (Human)
O75344
SwissProt (Mouse)
Q91XW8
SwissProt (Rat)
D3ZQF4
CAS Number
9007-83-4
Available Sizes
Curated Selection
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