Rabbit anti-FKBP4 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to FKBP4
Synonyms
FKBP52; Peptidyl-prolyl cis-trans isomerase FKBP4; PPIase FKBP4; 51 kDa FK506-binding protein; FKBP51; 52 kDa FK506-binding protein; 52 kDa FKBP; FKBP-52; 59 kDa immunophilin; p59; FK506-binding protein 4; FKBP-4; FKBP59; HSP-binding immunophilin; HBI; Immunophilin FKBP52; Rotamase
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human FKBP4
Clonality
Polyclonal
Applications
WB, IHC, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of FKBP4 expression in Hela (A), Jurkat (B), MCF7 (C), mouse testis (D) whole cell lysates.
IHC description
Immunohistochemical analysis of FKBP4 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of FKBP4 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
2288
Gene ID (Mouse)
14228
Gene ID (Rat)
260321
SwissProt (Human)
Q02790
SwissProt (Mouse)
P30416
SwissProt (Rat)
Q9QVC8
CAS Number
9007-83-4
Available Sizes
Curated Selection
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