SingleFlowEx CD25 PE-Cy™5

SingleFlowEx is fluorochrome-labeled monoclonal antibody single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine human clinical samples. SingleFlowEx can be used in various multicolor combinations. Laboratory shall establish the SingleFlowEx performance characteristics in conjunction with other reagents in normal and pathological samples.

Product Specifications

Certification

RUO (GMP)

Clone

MEM-181

Applications

FC (QC tested)

Concentration

0.020 mg/mL

Format

PE-Cy

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Components

1 vial of monoclonal antibody reagent.The content (1 mL) is sufficient for min. 100 Tests.

References & Citations

*Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May;19 (5) :675-84., URL: http://www.ncbi.nlm.nih.gov/pubmed/17446208?itool=, *Černý V, Hrdý J, Novotná O, Petrásková P, Boráková K, Kolářová L, Prokešová L: Distinct characteristics of Tregs of newborns of healthy and allergic mothers. PLoS One. 2018 Nov 26;13 (11) :e0207998., URL: https://www.ncbi.nlm.nih.gov/pubmed/30475891 , *Černý V, Novotná O, Petrásková P Hudcová K, Boráková K, Prokešová L, Kolářová L, Hrdý J: Lower functional and proportional characteristics of cord blood treg of male newborns compared with female newborns. Biomedicines 2021, 9, 170., URL: https://pubmed.ncbi.nlm.nih.gov/33572097/, *Use of this product has not been published., URL:

Other References

*Sotlar K, Horny HP, Simonitsch I, Krokowski M, Aichberger KJ, Mayerhofer M, Printz D, Fritsch G, Valent P: CD25 indicates the neoplastic phenotype of mast cells: a novel immunohistochemical marker for the diagnosis of systemic mastocytosis (SM) in routinely processed bone marrow biopsy specimens. Am J Surg Pathol. 2004 Oct;28 (10) :1319-25., URL: https://www.ncbi.nlm.nih.gov/pubmed/15371947?ordin, *Morris JC, Waldmann TA: Advances in interleukin 2 receptor targeted treatment. Ann Rheum Dis. 2000 Nov;59 Suppl 1:i109-14., URL: https://www.ncbi.nlm.nih.gov/pubmed/11053100?ordin, *Scheibenbogen C, Keilholz U, Richter M, Andreesen R, Hunstein W: The interleukin-2 receptor in human monocytes and macrophages: regulation of expression and release of the alpha and beta chains (p55 and p75) . Res Immunol. 1992 Jan;143 (1) :33-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/1565844?ordina, *Lai KN, Leung JC, Lai FM: Soluble interleukin 2 receptor release, interleukin 2 production, and interleukin 2 receptor expression in activated T-lymphocytes in vitro. Pathology. 1991 Jul;23 (3) :224-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/1838146?ordina