Rabbit anti-ERK1/2 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to ERK1/2
Synonyms
MAPK3; ERK1; PRKM3; Mitogen-activated protein kinase 3; MAP kinase 3; MAPK 3; ERT2; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; p44-MAPK; Microtubule-associated protein 2 kinase; p44-ERK1; MAPK1; ERK2; PRKM1; PRKM2; Mitogen-activated protein kinase 1; MAP kinase 1; MAPK 1; ERT1; Extracellular signal-regulated kinase 2; ERK-2; MAP kinase isoform p42; p42-MAPK; Mitogen-activated protein kinase 2; MAP kinase 2; MAPK 2
Host
Rabbit
Reactivity
H, M, R, B, D, P
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ERK1/2. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of ERK1/2 expression in HeLa (A), NIH3T3 (B), PC12 (C) whole cell lysates.
IHC description
Immunohistochemical analysis of ERK1/2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
5595
Gene ID (Mouse)
26417; 26413
Gene ID (Rat)
50689; 116590
SwissProt (Human)
P27361; P28482
SwissProt (Mouse)
Q63844; P63085
SwissProt (Rat)
P21708; P63086
CAS Number
9007-83-4
Available Sizes
Curated Selection
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