Rabbit anti-Emerin Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Emerin
Synonyms
EDMD; STA; Emerin
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human Emerin
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/20 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Emerin expression in Hela (A), Jurkat (B), PC3 (C) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of Emerin staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
2010
Gene ID (Mouse)
13726
Gene ID (Rat)
25437
SwissProt (Human)
P50402
SwissProt (Mouse)
O08579
SwissProt (Rat)
Q63190
CAS Number
9007-83-4
Available Sizes
Curated Selection
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