Rabbit anti-ELK1 (pS389) Antibody
Product Specifications
Background
Rabbit polyclonal antibody to ELK1 (pS389)
Synonyms
ETS domain-containing protein Elk-1
Host
Rabbit
Reactivity
H, M, R, Mk
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ELK1. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC, IP
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of ELK1 (pS389) expression in HT29 (A), HeLa (B), Jurkat (C) whole cell lysates.
IHC description
Immunohistochemical analysis of ELK1 (pS389) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
2002
Gene ID (Mouse)
13712
SwissProt (Human)
P19419
SwissProt (Mouse)
P41969
CAS Number
9007-83-4
Available Sizes
Curated Selection
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