Rabbit anti-EIF5 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to EIF5
Synonyms
Eukaryotic translation initiation factor 5; eIF-5
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human EIF5
Clonality
Polyclonal
Applications
WB, IHC, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of EIF5 expression in MCF7 (A), Hela (B) whole cell lysates.
IHC description
Immunohistochemical analysis of EIF5 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of EIF5 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
1983
Gene ID (Mouse)
102641436;217869
Gene ID (Rat)
56783
SwissProt (Human)
P55010
SwissProt (Mouse)
P59325
SwissProt (Rat)
Q07205
CAS Number
9007-83-4
Available Sizes
Curated Selection
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