Rabbit anti-EHHADH Antibody
Product Specifications
Background
Rabbit polyclonal antibody to EHHADH
Synonyms
ECHD; Peroxisomal bifunctional enzyme; PBE; PBFE
Host
Rabbit
Reactivity
H, M, R
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human EHHADH. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of EHHADH expression in HepG2 (A), HEK293T (B), A549 (C) whole cell lysates.
IHC description
Immunohistochemical analysis of EHHADH staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
1962
Gene ID (Mouse)
74147
Gene ID (Rat)
171142
SwissProt (Human)
Q08426
SwissProt (Mouse)
Q9DBM2
SwissProt (Rat)
P07896
CAS Number
9007-83-4
Available Sizes
Curated Selection
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