Rabbit anti-EEF1D Antibody
Product Specifications
Background
Rabbit polyclonal antibody to EEF1D
Synonyms
EF1D; Elongation factor 1-delta; EF-1-delta; Antigen NY-CO-4
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human EEF1D
Clonality
Polyclonal
Applications
WB, IHC, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of EEF1D expression in SHSY5Y (A), Jurkat (B), A549 (C), mouse spleen (D) whole cell lysates.
IHC description
Immunohistochemical analysis of EEF1D staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of EEF1D staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
1936
Gene ID (Mouse)
66656
Gene ID (Rat)
300033
SwissProt (Human)
P29692
SwissProt (Mouse)
P57776
SwissProt (Rat)
Q68FR9
CAS Number
9007-83-4
Available Sizes
Curated Selection
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