Rabbit anti-EAF2 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to EAF2
Synonyms
TRAITS; ELL-associated factor 2; Testosterone-regulated apoptosis inducer and tumor suppressor protein
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human EAF2
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of EAF2 expression in HepG2 (A), A431 (B), mouse kidney (C), rat kidney (D) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of EAF2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
55840
Gene ID (Mouse)
106389
Gene ID (Rat)
266787
SwissProt (Human)
Q96CJ1
SwissProt (Mouse)
Q91ZD6
SwissProt (Rat)
Q811X5
CAS Number
9007-83-4
Available Sizes
Curated Selection
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