Rabbit anti-DNase I Antibody
Product Specifications
Background
Rabbit polyclonal antibody to DNase I
CAS Number
9007-83-4
Synonyms
DNL1; DRNI; Deoxyribonuclease-1; Deoxyribonuclease I; DNase I; Dornase alfa
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human DNase I
Clonality
Polyclonal
Applications
WB, IHC, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
Host or Source
Rabbit
WB Description
Western blot analysis of DNase I expression in HT29 (A), mouse testis (B) whole cell lysates.
IHC description
Immunohistochemical analysis of DNase I staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of DNase I staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
1773
Gene ID (Mouse)
13419
Gene ID (Rat)
25633
SwissProt (Human)
P24855
SwissProt (Mouse)
P49183
SwissProt (Rat)
P21704
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