Rabbit anti-DNA Polymerase beta Antibody
Product Specifications
Background
Rabbit polyclonal antibody to DNA Polymerase beta
Synonyms
DNA polymerase beta
Host
Rabbit
Reactivity
H, M, R, B, C
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human DNA Polymerase beta. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of DNA Polymerase beta expression in HeLa (A), NIH3T3 (B), rat brain (C) whole cell lysates.
IHC description
Immunohistochemical analysis of DNA Polymerase beta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
5423
Gene ID (Mouse)
18970
Gene ID (Rat)
29240
SwissProt (Human)
P06746
SwissProt (Mouse)
Q8K409
SwissProt (Rat)
P06766
CAS Number
9007-83-4
Available Sizes
Curated Selection
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