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Rabbit anti-Decorin Antibody

Product Specifications

Background

Rabbit polyclonal antibody to Decorin

Synonyms

SLRR1B; Decorin; Bone proteoglycan II; PG-S2; PG40

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human Decorin

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1 mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of Decorin expression in mouse heart (A), mouse liver (B) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of Decorin staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

1634

Gene ID (Mouse)

13179

Gene ID (Rat)

29139

SwissProt (Human)

P07585

SwissProt (Mouse)

P28654

SwissProt (Rat)

Q01129

CAS Number

9007-83-4

Available Sizes

Curated Selection

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