Rabbit anti-DAP Antibody
Product Specifications
Background
Rabbit polyclonal antibody to DAP
CAS Number
9007-83-4
Synonyms
DAP1; Death-associated protein 1; DAP-1
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human DAP
Clonality
Polyclonal
Applications
WB, IHC, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/20 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
Host or Source
Rabbit
WB Description
Western blot analysis of DAP expression in HepG2 (A), Hela (B), NIH3T3 (C), rat testis (D) whole cell lysates.
IHC description
Immunohistochemical analysis of DAP staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of DAP staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
1611
Gene ID (Mouse)
223453
Gene ID (Rat)
64322
SwissProt (Human)
P51397
SwissProt (Mouse)
Q91XC8
SwissProt (Rat)
Q9QX67
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