Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

Rabbit anti-Cytokeratin 5 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to Cytokeratin 5

Synonyms

Keratin, type II cytoskeletal 5; 58 kDa cytokeratin; Cytokeratin-5; CK-5; Keratin-5; K5; Type-II keratin Kb5

Host

Rabbit

Reactivity

H, M, R, B, P

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Cytokeratin 5. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1 mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of Cytokeratin 5 expression in DU145 (A), HeLa (B), MCF7 (C) whole cell lysates.

IHC description

Immunohistochemical analysis of Cytokeratin 5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of Cytokeratin 5 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .

Gene ID (Human)

3852

Gene ID (Mouse)

110308

Gene ID (Rat)

369017

SwissProt (Human)

P13647

SwissProt (Mouse)

Q922U2

SwissProt (Rat)

Q6P6Q2

CAS Number

9007-83-4

Available Sizes

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

Rat Troponin T Type 2, Cardiac (TNNT2) ELISA Kit
EKN49023-01 48 Tests

Rat Troponin T Type 2, Cardiac (TNNT2) ELISA Kit

Ask
View Details
Rat Troponin T Type 2, Cardiac (TNNT2) ELISA Kit
EKN49023-02 96 Tests

Rat Troponin T Type 2, Cardiac (TNNT2) ELISA Kit

Ask
View Details
Rat Troponin T Type 2, Cardiac (TNNT2) ELISA Kit
EKN49023-03 5x 96 Tests

Rat Troponin T Type 2, Cardiac (TNNT2) ELISA Kit

Ask
View Details
SR-3306
BA6464-10 10 mg

SR-3306

Ask
View Details
Recombinant Myotis myotis Cytochrome b (MT-CYB), partial
MBS7097497 Inquire

Recombinant Myotis myotis Cytochrome b (MT-CYB), partial

Ask
View Details