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Rabbit anti-Cytokeratin 20 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to Cytokeratin 20

Synonyms

Keratin, type I cytoskeletal 20; Cytokeratin-20; CK-20; Keratin-20; K20; Protein IT

Host

Rabbit

Reactivity

H, M, R, B, P

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Cytokeratin 20. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1 mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of Cytokeratin 20 expression in A549 (A), NS-1 (B), mouse kidney (C), rat kidney (D) whole cell lysates.

IHC description

Immunohistochemical analysis of Cytokeratin 20 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of Cytokeratin 20 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .

Gene ID (Human)

54474

Gene ID (Mouse)

66809

Gene ID (Rat)

286912

SwissProt (Human)

P35900

SwissProt (Mouse)

Q9D312

SwissProt (Rat)

P25030

CAS Number

9007-83-4

Available Sizes

Curated Selection

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