Rabbit anti-Cytochrome P450 46A1 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Cytochrome P450 46A1
Synonyms
CYP46; Cholesterol 24-hydroxylase; CH24H; Cytochrome P450 46A1
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human Cytochrome P450 46A1
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Cytochrome P450 46A1 expression in MCF7 (A), mouse brain (B), rat liver (C) whole cell lysates.
IHC description
Immunohistochemical analysis of Cytochrome P450 46A1 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
10858
Gene ID (Mouse)
13116
SwissProt (Human)
Q9Y6A2
SwissProt (Mouse)
Q9WVK8
CAS Number
9007-83-4
Available Sizes
Curated Selection
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