Rabbit anti-Cyclin D2 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Cyclin D2
CAS Number
9007-83-4
Synonyms
G1/S-specific cyclin-D2
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human Cyclin D2
Clonality
Polyclonal
Applications
WB, IHC, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
Host or Source
Rabbit
WB Description
Western blot analysis of Cyclin D2 expression in SW480 (A), NIH3T3 (B), mouse brain (C), mouse lung (D), rat heart (E) whole cell lysates.
IHC description
Immunohistochemical analysis of Cyclin D2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of Cyclin D2 staining in SW480 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
894
Gene ID (Mouse)
12444
Gene ID (Rat)
64033
SwissProt (Human)
P30279
SwissProt (Mouse)
P30280
SwissProt (Rat)
Q04827
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