Rabbit anti-CTCF Antibody
Product Specifications
Background
Rabbit polyclonal antibody to CTCF
Synonyms
Transcriptional repressor CTCF; 11-zinc finger protein; CCCTC-binding factor; CTCFL paralog
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human CTCF
Clonality
Polyclonal
Applications
WB, IHC, ChIP
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200), ChIP (1/20 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of CTCF expression in Hela (A), PC3 (B), COS7 (C) whole cell lysates.
IHC description
Immunohistochemical analysis of CTCF staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
10664
Gene ID (Mouse)
13018
Gene ID (Rat)
83726
SwissProt (Human)
P49711
SwissProt (Mouse)
Q61164
SwissProt (Rat)
Q9R1D1
CAS Number
9007-83-4
Available Sizes
Curated Selection
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