Rabbit anti-c-Raf/B-RAF (pT491/599) Antibody
Product Specifications
Background
Rabbit polyclonal antibody to c-Raf/B-RAF (pT491/599)
CAS Number
9007-83-4
Synonyms
RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1; BRAF1; RAFB1; Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1
Host
Rabbit
Reactivity
H, M, R, B, C
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human c-Raf/B-RAF. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
Host or Source
Rabbit
WB Description
Western blot analysis of c-Raf/B-RAF (pT491/599) expression in HEK293T (A), HeLa (B) whole cell lysates.
IHC description
Immunohistochemical analysis of c-Raf/B-RAF (pT491/599) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
5894; 673
Gene ID (Mouse)
110157; 109880
Gene ID (Rat)
24703
SwissProt (Human)
P04049; P15056
SwissProt (Mouse)
Q99N57; P28028
SwissProt (Rat)
P11345
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