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Rabbit anti-c-RAF Antibody

Product Specifications

Background

Rabbit polyclonal antibody to c-RAF

Synonyms

RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1

Host

Rabbit

Reactivity

H, M, R, B, C, P

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human c-RAF. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1 mg/mL

Dilution

WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of c-RAF expression in A431 (A), HeLa (B), RAW264.7 (C), C6 (D) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of c-RAF staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

5894

Gene ID (Mouse)

110157

Gene ID (Rat)

24703

SwissProt (Human)

P04049

SwissProt (Mouse)

Q99N57

SwissProt (Rat)

P11345

CAS Number

9007-83-4

Available Sizes

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