Rabbit anti-CPT1A Antibody
Product Specifications
Background
Rabbit polyclonal antibody to CPT1A
Synonyms
CPT1; Carnitine O-palmitoyltransferase 1, liver isoform; CPT1-L; Carnitine O-palmitoyltransferase I, liver isoform; CPT I; CPTI-L; Carnitine palmitoyltransferase 1A
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human CPT1A
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of CPT1A expression in HL60 (A), THP1 (B), BT474 (C) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of CPT1A staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
1374
Gene ID (Mouse)
12894
Gene ID (Rat)
25757
SwissProt (Human)
P50416
SwissProt (Mouse)
P97742
SwissProt (Rat)
P32198
CAS Number
9007-83-4
Available Sizes
Curated Selection
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