Rabbit anti-COX2 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to COX2
Synonyms
COII; COXII; MTCO2; Cytochrome c oxidase subunit 2; Cytochrome c oxidase polypeptide II
Host
Rabbit
Reactivity
H, M, R
Immunogen
KLH-conjugated synthetic peptide of human COX2
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of COX2 expression in Hela (A), MCF7 (B), Jurkat (C), mouse brain (D), rat brain (E) whole cell lysates.
IHC description
Immunohistochemical analysis of COX2 staining in human stomach formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
4513
Gene ID (Mouse)
17709
SwissProt (Human)
P00403
SwissProt (Mouse)
P00405
SwissProt (Rat)
P00406
CAS Number
9007-83-4
Available Sizes
Curated Selection
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